Authors
L. W. Seeb,
A. Antonovich,
M. A. Banks,
T. D. Beacham,
M. R. Bellinger,
S. M. Blankenship,
M. R. Campbell,
N. A. Decovich,
J. C. Garza,
C.M. Guthrie III,
T. A. Lundrigan,
P. Moran,
S. R. Narum,
J. J. Stephenson,
K. J. Supernault,
D. J. Teel,
W. D. Templin,
J. K. Wenburg,
S. F. Young, and
C. T. Smith
Report Reference
#Fisheries
Vol. 32, No. 11:
540-549
Publication Date
November
2007
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Development
of a Standardized DNA Database for Chinook Salmon
Abstract |
| An international
multi-laboratory project was conducted to develop a standardized DNA
database for Chinook salmon (Oncorhynchus tshawytscha). this
project was in response to the needs of the Chinook Technical Committee
of the Pacific Salmon Commission to identify stock composition of
Chinook salmon caught in fisheries during their oceanic migrations.
Nine genetics laboratories identified 13 microsatellite loci that
could be reproducibly assayed in each of the laboratories. To test
that the loci were reproducible among laboratories, blind tests were
conducted to verify scoring consistency for the nearly 500 total alleles.
Once standardized, a dataset of over 16,000 Chinook salmon representing
110 putative populations was constructed ranging throughout the area
of interest of the Pacific Salmon Commission from southeast Alaska
to the Sacramento River in California. The dataset differentiates
the major known genetic lineages of Chinook salmon and provides a
tool for genetic stock identification of samples collected from mixed
fisheries. A diverse group of scientists representing the disciplines
of fishery management, genetics, fishery administration, population
dynamics, and sampling theory are now developing recommendations for
the integration of these genetic data into ocean salmon management. |
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